Part:BBa_K933001:Design
leader sequence with threonine repeats. mCherry and GFP reporters
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 66
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 66
Illegal NheI site found at 94
Illegal NheI site found at 117
Illegal NotI site found at 72 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 66
Illegal BamHI site found at 197 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 66
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 66
Illegal XbaI site found at 81 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was made by digesting out the orginal repeat sequence in BBa_K933002 at BamHI and HindIII and ligating in the threonine 6 repeat of ACT. The repeat sequence was made from annealed oligos.
Sequence data provided for: EcoRI, XbaI, J23100 Promoter, RBS34, leader sequence, BamHI, Threonine ACT repeat, HindIII
The part still has the Promoter, RBS34, leader sequence, repeat sequence (specifically Threonine ACT 6 repeats) mcherry, RBS30, and GFP. Built in sPB1C3 also.
Source
This piece was already partially assembled by a Ph.D student working in the Richard lab at Penn State named Mike Speer. Additional work was performed to perfect the construct with an alternate RBS from the registry. Repeat sequences were ordered as ssDNA oligos.